Creation of tools for the investigation of mechanisms involved in oxidative damage to DNA and RNA

Oxidative stress is one of the main area of focus in the Bryant-Friedrich lab.

Oxidative stress is one of the major contributors to the endogenous exposome. Although oxidative stress has been associated with the etiology of various diseases and conditions, the individual contributions of damaged lesions is yet being investigated. Using the tools of organic chemistry, we independently and site specifically generate lesions resulting from oxidative stress to study their fate and their contribution to disease states as potential biomarkers.
We are looking for students willing to engage in multistep organic synthesis of nucleoside analogs to determine their contribution to the endogenous exposome.

Successful completion of Organic chemistry lectures and labs (A or B grades)
Ability to work collaboratively within a team environment
Must be able to contribute a minimum of 10 hours per week to the research

The project is ongoing in the Bryant-Friedrich lab.

Perform multistep organic synthesis projects based on established lab procedures
Utilize thorough literature review to optimize lab protocols and procedures
Employ chromatographic techniques (TLC, LC, etc...) to isolate and characterize compounds
Characterize synthetic compounds using NMR, Mass Spec
Prepare weekly status reports
Prepare monthly project updates in a suitable format for publications including detailed experimental procedures, discussions and illustrations
Attend and contribute the team group meetings as scheduled

November 3, 2021